| 1. | Codon usage in genes coding for proteins with different folding types
不同折叠类型蛋白编码基因的密码子使用 |
| 2. | Expression of gene coding mature peptide of sag1 of toxoplasma gondii in yeast pichia methanolica
1成熟蛋白编码区基因在甲醇酵母中的初步表达 |
| 3. | Expression and significance of products that multi - drug resistance associated genes and apoptosis genes coded in non - small cell lung carcinoma
2基因在非小细胞肺癌中的表达和相关性 |
| 4. | According to the southern blot results , a 6 . 5 kb bamh i restriction fragment containing the gene coding for hal c8 was cloned and sequenced
Halcs的开放阅读框为849个核昔酸，编码283个氨基酸;它必须要剪切掉n端的197个氨基酸才能成为成熟的蛋白。 |
| 5. | One solobp ecor i fragment containing phospholipase gene was isolated . further sequence analysis and subcloning revealed a 963bp phla . gene coding a 320aa phospholipase phl with deduced molecular weight of 33kd
通过测序及亚克隆分析，发现一个磷酯酶的基因phla ，长度为963bp ，预测编码一个由320个氨基酸组成，分子量为33kd的磷酯酶phl 。 |
| 6. | The association of larval and adult stages of ecologically important caddisfly ( insecta : trichoptera ) using mitochondrial dna sequences larval and adult amplification fragments were cloned and sequenced . dna sequences were aligned using clustal x software ( gene codes corporation )
线粒休dna一coi 、 u基因序列在毛翅目成幼虫联系中的应用研究扩增并测定了4种鳞石蛾的成、幼虫的mtdnacoi 、 coh及trna一leu基因序歹‘ j 。 |
| 7. | Genes coding mature peptide of igfs were achieved by pcr using another pair of oligo - nucleotide primers to induce to the suitable restriction enzyme site , and the igf - i product of pcr contains 230 base pairs . igf - ii contains 219 base pairs . 3
各另外设计一对特异性pcr引物，导入适当限制性内切酶切点，以上述连有目的基因的克隆载体为模板，采用pcr方法扩增基因片段，获得长度约230bp的igf -和219bp的igf -成熟肽基因序列。 |
| 8. | The results showed that the f fragment , 728bp in length , could be a new gene with a little homology to the genes coding for polyketide synthetase or fatty - acid synthetase and the b fragment , about 4kb in length , is inferred to have repeat sequences around tn5 insertion site , in which there is homology to the wa 314 right arm of the high - pathogeniciry island of yersinia enterocolitica . to reveal any pathogenicity of enterobacter cloacae b8 and its mutated strains b8b and b8f to animals , the experiment with mice was carried out
结果显示， f片段长度为728bp ，与现有生物数据库的blast比较分析，发现该序列仅有局部短于1oobp的区域与polyketide合成酶基因或与脂肪酸合成酶基因有低的同源性，推测为一新基因； b片段长约4kb ，序列拼接结果推测靠近tn5插入位点部位有重复序列，对b片段tn5远端的部分序列进行blast比较，发现它与小肠结肠炎耶尔森氏菌的强毒力岛有一定的同源性。 |
| 9. | In addition , some ntfs could induce the development of normal vision pathway , and even help the injured neuron to regenerate synapse . in this study , the genes coding for mature nts and gdnf were cloned through polymerase chain reaction . after sequencing , alignment showed the ntfs were highly conserved
研究表明，神经营养因子对癫痫造成的神经元损伤具有保护及再生作用，而对于视神经通路的发育和分化，以及受损视觉通路的修复也饶刚：大熊独和朱赐神经营养因子基因家族的克隆、表达及活性鉴定起到关键作用。 |
| 10. | Pcr products were inserted into pbluescriptsk using psti and xhoi . the element bearing resistance to streptomycin ( sm ) and spectinomycin ( sp ) was excised from php45 and inserted into gene coding region . the disrupted gene was isolated as a pstl and xhol fragment and cloned into prl271
首先将pcr扩增片段( 1 . 5kb )利用pst和xho插入常规克隆载体pbluescriptsk ，利用基因内部合适的酶切位点插入sp sm抗性片段，再利用pst和xho位点将已插入抗性基因的pcr片段，转入整合载体prl271中获得体外基因中断，最后利用三亲本杂交获得体内突变。 |
